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The volumes detail experiments conducted by Professor Ray Dixon FRS during his PhD and early postdoctoral period at the ARC Unit of Nitrogen Fixation, University of Sussex. The volumes from 1969-1976 detail the experiments which formed the basis of three papers published in Nature during the 1970s. 1. Dixon, R.A., and Postgate, J.R. (1971). Transfer of nitrogen-fixation genes by conjugation in Klebsiella pneumoniae. Nature 234, 47-48. 2. Dixon, R., and Postgate, J.R. (1972). Genetic transfer of nitrogen fixation from Klebsiella pneumoniae to Escherichia coli. Nature 237, 102-103. 3. Dixon, R., Cannon, F., and Kondorosi, A. (1976). Construction of a P plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae. Nature 260, 268-271. Publication 2 is particularly notable, because it reported the first transfer of an entire nif gene cluster into an organism that does not fix nitrogen, thus engineering the first synthetic diazotroph. This work was conducted prior to the advent of recombinant DNA technology and has been highly cited. Publication 3 was also a landmark in the field, as it reported the engineering of a plasmid that enabled genetic characterisation and cloning of nitrogen fixation genes. It has formed the basis of many recent synthetic biology advances, including the engineering of nitrogenase components in plant mitochondria. Most volumes are organised chronologically, but the first volume is arranged into sections, which group details of methods used, and individual experiments separately.